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选用安徽太湖县二化螟 (ChilosuppressalisWalker)自然种群 ,用 6种药剂对 6龄幼虫进行毒力测定 ,并与国内外已知敏感品系的LD50 值比较 ,在证实了该种群具有较高的敏感水平后 ,经室内纯化和连续饲养近 2 0代 ,育成相对敏感品系(TH S)。为提高试验准确性 ,简化操作 ,使用 4龄幼虫进行抗药性研究。用于测试的 4龄幼虫形态特征为头宽 (0 86±0 0 4)mm ,体重 (8 5± 1 5 )mg ,前胸与头宽之比 1 19± 0 0 4,体长 (10 0± 0 8)mm。在详细观察 4龄幼虫发育历期和形态特征后 ,选用该龄幼虫 ,建立了包括沙蚕毒素、有机磷、杂环类和生物制剂等类的 6种杀虫剂的相对敏感基线。
The natural population of Chilosuppressalis Walker in Taihu County of Anhui Province was selected and the virulence of 6th instar larvae was tested with 6 kinds of pesticides. Compared with the LD50 values of known sensitive strains at home and abroad, it was confirmed that the population was highly sensitive After the indoor purification and continuous feeding of nearly 20 generations, bred relatively sensitive strains (TH S). In order to improve the test accuracy and simplify the operation, 4th instar larvae were used for drug resistance study. The morphological characteristics of 4th instar larvae used for testing were head width (86 ± 0 0 4) mm, body weight (85 ± 1 5) mg, ratio of anterior chest to head width 1 19 ± 0 0 4, body length 0 ± 0 8) mm. After observing the developmental history and morphological characteristics of the 4th instar larvae, we selected the larvae of this instar to establish the relative sensitive baseline of six insecticides, including Nereis, organophosphates, heterocycles and biological agents.