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目的探讨TNF-α对小鼠卵母细胞成熟过程纺锤体组装及染色体排列的影响。方法收集小鼠未成熟卵母细胞,在含不同浓度TNF-α(0,5,10,100ng/ml)的培养液中进行培养3、9、12h,所有卵母细胞体外培养12h后固定观察纺锤体及染色体分布情况。结果本研究结果表明当TNF-α浓度为5或10ng/ml时,与对照组相比,纺锤体组装正常及染色体排列正常的卵母细胞比例无明显差异(P>0.05)。当TNF-α浓度升高至100ng/ml时,与低浓度组及对照相比,卵母细胞纺锤体组装及染色体排列均呈现明显异常,差异有统计学意义(P<0.05)。结论一定浓度的TNF-α影响卵母细胞纺锤体的组装及染色体的排列从而影响卵的质量。
Objective To investigate the effect of TNF-α on spindle assembly and chromosome arrangement in mouse oocyte maturation. Methods Mature immature oocytes were collected and cultured in culture medium containing different concentrations of TNF-α (0, 5, 10 and 100 ng / ml) for 3, 9 and 12 h. All oocytes were cultured in vitro for 12 h. Body and chromosome distribution. Results The results of this study showed that there was no significant difference (P> 0.05) in spindle assembly and normal chromosome arrangement when the concentration of TNF-α was 5 or 10 ng / ml. When the concentration of TNF-α was increased to 100 ng / ml, the oocyte spindle assembly and chromosome arrangement showed obvious abnormalities compared with the low concentration group and the control group, the difference was statistically significant (P <0.05). Conclusion A certain concentration of TNF-α affects the oocyte spindle assembly and chromosomal arrangement to affect egg quality.