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目的 探索钩藤碱对大鼠脑缺血损伤的机制。方法 采用大鼠大脑中动脉缺血模型 ,利用微透析方法收集正常及脑缺血后不同时间点纹状体和海马细胞外液 (透析液 )。经反相高效液相电化学法检测其单胺类神经递质含量的变化。结果 大鼠脑缺血后纹状体和海马细胞外液中 5 羟吲哚乙酸 (5 HIAA)、3,4 二羟苯酰乙酸 (DOPAC)和高香草酸(HVA)含量下降 ,去甲肾上腺素 (NE)含量上升 ,钩藤碱能升高脑缺血后细胞外液 5 HIAA ,DOPAC和HVA的含量 ,降低NE的含量。结论 钩藤碱能调节脑缺血大鼠纹状体内和海马单胺类神经递质及代谢物的含量
Objective To explore the mechanism of rhynchophylline on cerebral ischemic injury in rats. METHODS: Rat middle cerebral artery ischemia model was used to collect extracellular fluid (dialysis fluid) from striatum and hippocampus at different time points after normal and cerebral ischemia by means of microdialysis. The change of monoamine neurotransmitter content was detected by RP-HPLC. Results The levels of 5-HIAA, DOPAC and HVA in the extracellular fluid of striatum and hippocampus after cerebral ischemia in rats were decreased. With the increase of NE content, rhynchophylline can increase the content of 5 HIAA, DOPAC and HVA in extracellular fluid after cerebral ischemia, and decrease the content of NE. Conclusion Rhynchophylline can regulate the content of monoamine neurotransmitters and metabolites in the striatum and hippocampus of rats with cerebral ischemia