用细胞微点法预选肿瘤病人血清抗体,取其淋巴结细胞进行ERV转化,发现抗体反应强者,其转化的淋巴细胞分泌阳性抗体率高。用此法获得分泌抗乳癌人单抗或多抗的淋巴母细胞系共14株。 Serum antibodies of tumor patients were pre-selected by the cell micro-spot method, and the lymph node cells were taken for ERV transformation. It was found that the antibody reaction was strong and the rate of positive antibody secreted by the transformed lymphocytes was high. In this way, a total of 14 lymphoblastoid cell lines secreting anti-breast cancer human monoclonal antibodies or polyclonal antibodies were obtained.