亚洲鲎血细胞溶解物先经CM-Sepharose CL-6B分级洗脱,再经Dextran-Sepharose CL-6B亲和层析纯化凝固蛋白原。用此法纯化的凝固蛋白原免疫家兔产生抗血清,并用火箭免疫电泳法测定鲎血细胞溶解物与内毒素反应后的凝固蛋白原残余量,根据残余凝固蛋白原量与参加反应内毒素量呈负相关关系,建立了定量测定内毒素的火箭免疫电泳鲎试验法。 Ascochyta sephacryl was fractionated by CM-Sepharose CL-6B and purified by Dextran-Sepharose CL-6B affinity chromatography. The purified coagulogen was used to immunize rabbits to produce antiserum, and rocket immunoelectrophoresis was used to determine the residual amount of coagulation protein after the reaction of hemagglutinated lysate and endotoxin. According to the amount of residual coagulation protein and the amount of endotoxin involved Negative correlation, the establishment of a quantitative determination of endotoxin rocket immunoelectrophoresis 鲎 test.