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目的 研究硫酸多糖 916 (PS916 )对TNFa、IL 1β和H2 O2 诱导ECV30 4细胞产生一氧化氮 (NO)的影响。方法 用Griess试剂测定ECV30 4细胞产生的NO ,用MTT法测定细胞的增殖 ,用荧光法测定NO合酶的活性。结果 4 0ng/mlTNFa和 4 0ng/mlIL 1β使ECV30 4细胞NO产生下降 ,0 1mmol/LH2 O2 使NO的产生增加。PS916剂量依赖性增加TNFa、IL 1β诱导的ECV30 4细胞NO的产生 ,降低H2 O2 诱导ECV30 4细胞产生的NO。TNFa和H2 O2 抑制ECV30 4细胞的增殖 ,而IL 1β对ECV30 4细胞的增殖没有明显影响。PS916剂量依赖性的抑制TNFa和H2 O2 的作用 ,增加存活细胞数。在体外 ,PS916对细胞的NO合酶无明显的影响。结论 PS916在体外实验中对细胞因子和H2 O2 引起的ECV30 4细胞损伤有拮抗作用 ,对细胞表现出明显的保护作用。
Objective To investigate the effect of polysaccharide sulfate 916 (PS916) on the production of nitric oxide (NO) in ECV304 cells induced by TNFa, IL-1β and H2O2. Methods The NO produced by ECV304 cells was assayed by Griess reagent. The proliferation of ECV304 cells was measured by MTT assay. The activity of NO synthase was determined by fluorescence assay. Results 4 0 ng / ml TNFa and 4 0 ng / ml IL 1β decreased NO production in ECV30 4 cells, while 0 1 mmol / LH 2 O2 increased NO production. PS916 dose-dependently increased TNFa and IL-1βinduced NO production in ECV304 cells and decreased NO induced by H2O2 in ECV304 cells. TNFa and H2O2 inhibited the proliferation of ECV304 cells, whereas IL1β had no significant effect on the proliferation of ECV304 cells. PS916 dose-dependently inhibited the effects of TNFa and H2O2, increasing the number of viable cells. In vitro, PS916 had no significant effect on NO synthase in cells. Conclusion PS916 can antagonize the injury of ECV304 cells induced by H2O2 in vitro and in vitro, and it shows obvious protective effect on cells.