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探讨了PCR鉴定中模板来源、DNA聚合酶、引物的选择等三方面的策略,并从特异复合PCR鉴定、共同复合PCR鉴定、两步复合PCR鉴定、特异PCR鉴定、PCR-RFLP鉴定等五个角度对其进展作了回顾与展望。
The strategy of template source, DNA polymerase and primer selection in PCR identification was discussed. Five strategies including specific hybridization PCR identification, common compound PCR identification, two-step multiplex PCR identification, specific PCR identification and PCR-RFLP identification From the perspective of its progress made a review and outlook.