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目的研究氧化石墨烯(GO)PEG化前、后对L929细胞毒性的影响。方法将羧基化后的GO分子通过酰胺键与双端氨基聚乙二醇4000(NH2-PEG4000-NH2)结合,用细胞染色法来考察小鼠成纤维细胞(L929)与GO或GO-PEG一同孵育后生存率与黏附性的变化,从而比较GO分子PEG化前、后细胞毒性的变化。结果成功制备了PEG化氧化石墨烯,在相同给药浓度与孵育时间的条件下,L929细胞与经过PEG4000修饰的GO分子孵育后的相对生存率与细胞毒性明显大于未经修饰者。结论 PEG化可显著降低GO的细胞毒性并减少对细胞黏附性的影响,其作用机制可能是因为PEG化可以通过拮抗细胞内氧化应激损伤而降低GO的细胞毒性。GO-PEG可以作为一种安全的药物载体运用于载药系统中。
Objective To investigate the effect of graphene oxide (GO) pretreatment on the cytotoxicity of L929 cells. Methods The carboxylated GO molecules were bound to the double-terminal amino polyethylene glycol 4000 (NH2-PEG4000-NH2) via amide bond. The cell staining method was used to investigate the effect of mouse fibroblasts (L929) and GO or GO-PEG After incubation, the changes of survival rate and adhesiveness were compared, and the changes of cytotoxicity before and after GO PEGylation were compared. Results PEGylated graphene oxide was successfully prepared. The relative survival rate and cytotoxicity of L929 cells incubated with PEG4000 modified GO molecules were significantly higher than those of unmodified ones under the same drug concentration and incubation time. Conclusion PEGylation can significantly reduce the cytotoxicity of GO and reduce the impact on cell adhesion. The possible mechanism is that PEGylation can reduce the cytotoxicity of GO by antagonizing intracellular oxidative stress. GO-PEG can be used as a safe drug carrier in drug delivery systems.