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目的观察红霉素对被动吸烟大鼠肺组织气肿样变的保护作用并初步探讨其作用机制。方法 40只SD大鼠随机分为正常对照组、模型组、红霉素组、桂龙咳喘宁对照组,每组10只;采用气管内注入脂多糖(LPS)及烟熏的混合刺激方法建立肺气肿大鼠模型,造模3周后进行药物干预,连续14 d。肺组织病理切片观察形态学改变并采用图像分析系统检测平均肺泡面积(MAA),平均肺泡数(MAN)和肺平均内衬间隔(MLI),免疫组化测定肺组织中MMP-2与TIMP-2的表达量,ELISA法测定血清中IL-10与TNF-α的浓度。结果模型组大鼠肺组织可见肺大泡形成,MAA明显增大,MAN明显减少以及MLI显著增大,且有明显的炎症细胞浸润以及血清中TNF-α的浓度明显升高,与正常组比较有显著性差异(P<0.05);红霉素组(100 mg.kg?1)未见肺大泡形成,炎症细胞浸润明显减轻,且能明显降低肺组织中MMP-2表达量与血清中TNF-α的浓度,并能明显的增加肺组织中TIMP-2的表达量与血清中IL-10的浓度,与模型组比较有显著性差异(P<0.05),与桂龙咳喘宁组(450 mg.kg?1)相比无显著性的差异。结论红霉素对被动吸烟大鼠肺组织气肿样变具有明显的保护作用。
Objective To observe the protective effect of erythromycin on the emphysematous changes of lung in passive smoking rats and to explore its mechanism. Methods Forty Sprague-Dawley rats were randomly divided into normal control group, model group, erythromycin group and Guilong Kechuanning control group, with 10 rats in each group. Mixed tracheal intratracheal instillation of lipopolysaccharide (LPS) Establishment of rat model of emphysema, 3 weeks after modeling for drug intervention, continuous 14 d. The pathological changes of lung tissue were observed and the average alveolar area (MAA), mean alveolar number (MAN) and mean lung lining interval (MLI) were detected by image analysis system. The expressions of MMP-2 and TIMP- 2 expression, ELISA method for the determination of IL-10 and TNF-α serum concentration. Results In the model group, the formation of pulmonary alveoli in the lung tissue was observed. The MAA was significantly increased, the number of MAN was significantly decreased, the MLI was significantly increased, and the infiltration of inflammatory cells and the concentration of TNF-α in serum were significantly increased. Compared with the normal group (P <0.05). Erythromycin group (100 mg · kg-1) showed no bullae formation and inflammatory cell infiltration, and significantly decreased the expression of MMP-2 in lung tissue TNF-α, and significantly increased the expression of TIMP-2 in lung tissue and the concentration of IL-10 in serum, which was significantly different from the model group (P <0.05) (450 mg.kg? 1) compared to no significant difference. Conclusion Erythromycin has a significant protective effect on emphysematous changes of lung tissue in passive smoking rats.