论文部分内容阅读
目的了解血清抗－ＨＩＶ阴性的静脉药瘾者肝组织是否存在前病毒ＨＩＶＤＮＡ。方法选择３８例抗－ＨＩＶ阴性的静脉药瘾者肝组织，１０例非静脉药瘾者肝组织，用巢式聚合酶链反应，分别在ＨＩＶｇａｇ、ｐｏｌ、ｅｎｖ三大结构区，用六对引物进行ＨＩＶＤＮＡ扩增。结果３８例中有３例同时在三个区域测到ＨＩＶＤＮＡ，１２例分别在二个区域、１７例在单一区域检测到ＨＩＶＤＮＡ，６例在ＨＩＶ三个区域均阴性。对照组１０例无一例检到ＨＩＶＤＮＡ。结论抗－ＨＩＶ阴性的静脉药瘾高危人群，存在潜在ＨＩＶ感染。提示潜在ＨＩＶ感染的检测及加强“窗口期”ＨＩＶ的监测十分重要
Objective To understand the existence of provirus HIVDNA in serum of anti-HIV-negative intravenous drug users. Methods Thirty-eight liver tissues of anti-HIV negative intravenous drug addicts and 10 non-intravenous drug addicts were selected. Nested polymerase chain reaction (PCR) was performed in three regions of HIVgag, pol and env respectively. Six pairs of primers HIVDNA amplification was performed. Results Three of the 38 patients had HIVDNA detected in three regions at the same time, 12 cases were in two regions, 17 cases were detected HIVDNA in a single region, and 6 cases were negative in all three regions of HIV. None of the 10 cases in the control group were detected HIVDNA. Conclusions There is potential HIV infection in anti-HIV-negative high-risk patients with intravenous drug addiction. Prompted detection of potential HIV infection and enhanced monitoring of “windowed” HIV are important