论文部分内容阅读
癌细胞腹腔种植及腹膜微转移是影响胃癌预后的重要因素 ,目前主要诊断方法是腹腔灌洗液细胞学检查 ,但敏感性较差。而端粒酶在恶性肿瘤细胞中的表达率极高 ,是诊断恶性肿瘤的可靠指标。我们用原位分子杂交技术 (ISH)测定胃癌术前术后腹腔灌洗液中细胞内端粒酶活性 ,检查腹腔中
Abdominal implantation of cancer cells and peritoneal micrometastasis is an important factor affecting the prognosis of gastric cancer. At present, the main diagnostic method is peritoneal lavage fluid cytology, but the sensitivity is poor. The high expression rate of telomerase in malignant cells is a reliable indicator of the diagnosis of malignant tumors. We use in situ hybridization (ISH) technique to measure intracellular telomerase activity in peritoneal lavage fluid before and after operation,