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本文提出以铜离子改性键合氨基柱和紫外检测器分离、测定氨基酸的高效液相色谱方法.研究了铜离子、醋酸缓冲液和己基磺酸盐浓度及pH值对氨基酸保留值和检测灵敏度的影响,探讨了氨基酸的分离和检测机理.
In this paper, a high performance liquid chromatography (HPLC) method was developed for the separation and determination of amino acids using copper ion modified bonded amino columns and UV detector.The effects of copper ion, acetate buffer and hexylsulfonate concentration and pH value on amino acid retention and detection sensitivity The effects of amino acids separation and detection mechanism.