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目的：研究肝细胞癌和肝硬变中ｂｃｌ－２蛋白表达与细胞凋亡的关系。方法：应用原位脱氧核糖核酸末端转移酶标记法和Ｓ－Ｐ免疫组化技术检测２８例肝硬变和３５例肝细胞癌组织中凋亡细胞的分布、密度及ｂｃｌ－２蛋白的表达。结果：肝细胞癌组织中凋亡细胞密度显著低于肝硬变，且随其恶性程度的增高呈逐渐降低趋势；凋亡细胞在肝硬变中多分布于假小叶周边区域。ｂｃｌ－２蛋白在肝细胞癌组织中的表达强度明显高于肝硬变，但表达的阳性率差异不明显。结论：ｂｃｌ－２通过其表达产物调控肝硬变和肝细胞癌中的细胞凋亡，在肝细胞癌发生中起重要作用，但ｂｃｌ－２蛋白并非细胞凋亡的唯一调控因素
Objective: To study the relationship between bcl-2 protein expression and apoptosis in hepatocellular carcinoma and liver cirrhosis. METHODS: The distribution and density of apoptotic cells and the expression of bcl-2 protein in 28 cases of cirrhosis and 35 cases of hepatocellular carcinoma were detected by in situ deoxyribonucleic acid terminal transferase labeling and S-P immunohistochemistry. RESULTS: The apoptotic cell density in hepatocellular carcinoma was significantly lower than that in cirrhosis, and gradually decreased along with the increase of its malignancy. Apoptotic cells were mostly distributed in the peripheral region of pseudolobular in cirrhosis. The expression level of bcl-2 protein in hepatocellular carcinoma was significantly higher than that in cirrhosis, but the positive rate of expression was not significant. CONCLUSION: bcl-2 regulates cirrhosis and apoptosis in hepatocellular carcinoma through its expression products and plays an important role in the development of hepatocellular carcinoma, but bcl-2 protein is not the only regulator of apoptosis.