选取大鼠第11天胚胎至成体脑58个,用Lewis改良的Koelle法,加以简化作乙酰胆硷酯酶(AChE)染色,配合用Nissl法作相应时期的染色。系统观察了大脑皮质组织发生中AChE的出现、分布部位及其消退的变化,发现这种变化与神经母细胞的迁移运动及其分化为神经元之间具有伴行的关系。在小脑发生过程中也观察到类似的现象。因此,作者分析在脑发生的早期,这种AChE的出现,和神经母细胞的迁移运动及其分化发育有关。 Fifty-one embryos were selected from the rat to 58 adult brains, which were reduced to acetylcholinesterase (AChE) staining by Lewis modified Koelle method and stained with the Nissl method for the corresponding period. The occurrence and distribution of AChE in the cerebral cortex were observed systematically. The changes of AChE were observed in relation to the migration and differentiation of neuroblasts and their differentiation into neurons. A similar phenomenon was observed during cerebellar processes. Therefore, the authors analyzed that in the early stages of brain development, the appearance of this AChE is related to the migration and differentiation of neuroblasts.