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一、转运核糖核酸甲基化核苷检测在肿瘤诊断上的应用本讲介绍肿瘤诊断的一个新领域,它涉及癌细胞里转运核糖核酸(tRNA)中的甲基化核苷问题。 1957年Guttman发现癌症患者尿里甲基化的嘌呤和嘧啶比正常者高两倍,它来自tRNA的降解而不是细胞的死亡。tRNA中经修饰的核苷酸含量很高,由于tRNA的转换率高,所以在尿中出现的甲基化嘌呤和嘧啶高。哺乳动物肝tRNA的转换率约为72小时,细胞中没有将甲基化核苷转变或修饰为核苷三磷酸的激酶,否则,修饰的核苷三磷酸会被核糖核酸聚
First, the application of RNA methylation nucleoside detection in the diagnosis of tumors This lecture introduces a new area of tumor diagnosis, which involves the issue of methylated nucleosides in the translocation of ribonucleic acid (tRNA) in cancer cells. In 1957, Guttman found that urinary methylated purines and pyrimidines in cancer patients were twice as high as those in normal subjects. It was derived from tRNA degradation rather than cell death. The content of modified nucleotides in tRNA is high, and methylation of purines and pyrimidines in urine is high due to the high conversion rate of tRNA. The mammalian liver tRNA turnover rate is approximately 72 hours. There is no kinase in the cell that converts or modifies methylated nucleosides into nucleoside triphosphates. Otherwise, the modified nucleoside triphosphates will be ribose-ribonucleotide.