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By employing the QCD factorization approach for the exclusive B → Vγ decays, we study the exclusive decay B → K*γ in the top-quark two-Higgs-doublet model (T2HDM). Within the considered parameter space, we find the following conclusions (a) A light charged-Higgs boson with a mass about 200 GeV is clearly excluded by the date of B → K*γ decay, and this lower limit is comparable with that from the inclusive B → Xsγ decay; (b) The theoretical predictions for CP asymmetry of B → K*γ in the T2HDM is always less than 1% in size; and (c) The isospin symmetry breaking for B → K*γ decay in the SM and T2HDM considered here is around 6% in size and well consistent with the data and the general expectations.