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目的 观察不同浓度异丙酚或硫喷妥钠对离体灌流大鼠心脏收缩力和心肌细胞质膜Ca2 + ATPase活性的影响。方法 ( 1)以含不同浓度 ( 10 μmol/L～ 10 0 0 μmol/L)异丙酚或硫喷妥钠的克 汉二氏重碳酸盐缓冲液 (KHB)灌流离体大鼠心脏 ,测定心率 (HR)、左室收缩压 (LVSP)、左室收缩压上升最大速率 ( +dp/dtmax)和左室舒张压下降最大速率 ( -dp/dtmax)。 ( 2 )参照文献制备大鼠心肌细胞质膜 ,观察不同浓度异丙酚或硫喷妥钠对质膜Ca2 + ATPase活性的影响。结果 随着药液浓度的升高 ,异丙酚或硫喷妥钠使LVSP、+dp/dtmax、-dp/dtmax逐渐降低 ,在等摩尔浓度的抑制作用异丙酚 >硫喷妥钠 ,高浓度的异丙酚或硫喷妥钠还可以减慢心率。异丙酚对质膜Ca2 + ATPase活性的影响呈双相 ,低浓度 (≤ 2 0 μmol/L)使酶的活性升高 ,而≥ 5 0 μmol/L的异丙酚使质膜Ca2 + ATPase活性下降 ,与基础值比较有统计学意义 (P <0 0 5 )。硫喷妥钠只有在浓度≥ 10 0mmol/L时对Ca2 + AT Pase活性有抑制作用。结论 异丙酚或硫喷妥钠呈浓度依赖地抑制心肌收缩和舒张功能 ,异丙酚呈双相地影响心肌质膜Ca2 + ATPase的活性 ,这可能是其抑制心肌舒张功能的原因之一
Objective To observe the effects of different concentrations of propofol or thiopental on cardiac contractility and myocardial Ca2 + ATPase activity in isolated perfused rat hearts. Methods (1) The isolated rat hearts were perfused with KHB containing different concentrations of propofol or thiopental sodium (KHB) at different concentrations (10 μmol / L ~ 100 μmol / L) HR, LVSP, + dp / dtmax and -dp / dtmax were measured. (2) The plasma membrane of rat myocardial cells was prepared with reference to the literature, and the effects of different concentrations of propofol or thiopental on plasma membrane Ca2 + ATPase activity were observed. Results Propofol or thiopental decreased the LVSP, + dp / dtmax and -dp / dtmax gradually with the increase of the concentration of propofol. The inhibitory effect of propofol> thiopental on the equimolar concentration was higher Concentration of propofol or thiopental may also slow heart rate. The effect of propofol on plasma membrane Ca2 + ATPase activity was biphasic. Low concentration (≤20 μmol / L) increased the activity of propofol, whereas ≥50 μmol / L propofol induced Ca2 + ATPase activity Decreased, compared with the baseline value was statistically significant (P <0 05). Thiopental only inhibited the activity of Ca2 + AT Pase when its concentration was ≥10 0mmol / L. Conclusions Propofol or thiopental inhibits myocardial contractile and diastolic function in a concentration-dependent manner. Propofol plays a dual role in affecting Ca2 + ATPase activity in the plasma membrane of the myocardium, which may be one of the reasons that it inhibits myocardial diastolic function