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目的:用高效液相色谱-电化学检测(HPLC-EC)或彗星分析两种方法测定60Coγ射线照射单核细胞DNA损伤水平的比较。方法:用碱性彗星分析与甲酰胺嘧啶-DNA-糖基酶(Fpg)和内切核酸酶(Endo)DNA-糖基酶相结合,估计修饰碱基与链断裂和碱-不稳定位点的量。用HPLC-EC法测定细胞内8-oxodGuo
OBJECTIVE: To compare the DNA damage of 60Co γ -ray irradiated mononuclear cells by HPLC-EC or comet assay. METHODS: Alkaline comet assay was used in combination with formamidine-DNA-glycosylase (Fpg) and endo-DNA-glycosylase to assess the effect of modified bases on strand breaks and base-labile sites The amount of. Determination of intracellular 8-oxodGuo by HPLC-EC method