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精液液化可能有多种酶参与,目前其生理和生化方面的原理尚不十分清楚.人精液射出后立即凝固,通常在5～10min或10～20min再液化.精液液化时间过长(大于30min)则视为液化异常.称“精液不液化症”或“精液迟缓液化症”.精液不液化症可能是引起不育或者生育力低下的原因之一.本文对24例精液不液化症的精液分别加透明质酸酶、α-淀粉酶及糜蛋白酶,促使液化.结果不液化精液加酶后液化时间都明显缩短,精子活力也有所增加.材料和方法一、实验材料透明质酸酶(江苏泰州制药厂)每支1500U,糜蛋白酶(上海生物化学制药厂)每支4000U.α-淀粉酶(美国Sigma公司)25U/mg.二、方法禁欲3～5d.手淫法采集精液.精液常规分析按WHO“精液常规分析实验室手册”所规定的方法进行.将一份不液化的精液(液化时间大于6h),分为四管.每管0.5ml或1.0ml,根据射出的精液总量而定.如射出量小于4ml则每管0.5ml精液.
Sperm liquefaction may have a variety of enzymes involved, the current physiological and biochemical aspects of the principle is not very clear.People sperm immediately after injection of solidification, usually in 5 ~ 10min or 10 ~ 20min and then liquefaction.Size liquefaction time is too long (more than 30min) It is considered abnormal liquefaction, said “semen liquefaction” or “semen liquefaction delay.” Seminal liquefaction may be caused by infertility or fertility is one of the reasons.This article on 24 cases of semen liquefaction semen, respectively Plus hyaluronidase, α-amylase and chymotrypsin to promote liquefaction.Results The liquefaction time of non-liquefied semen plus enzyme was significantly shortened, sperm motility also increased.Materials and Methods1, Materials hyaluronidase (Taizhou, Jiangsu Pharmaceutical Factory) Each 1500U, chymotrypsin (Shanghai Biochemical Pharmaceutical Factory) Each 4000U.α-amylase (Sigma, USA) 25U / mg. Second, the method of abstinence 3 ~ 5d. WHO sperm routine analytical laboratories manual method according to a non-liquefied semen (liquefaction time greater than 6h) is divided into four tubes each tube 0.5ml or 1.0ml, according to the total amount of semen injection If the shot is less than 4 ml each tube 0.5ml semen.