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传统的RT－PCR方法，要对模板RNA进行提取和纯化，以除去RNA酶和其它杂质，而且要对可能存在的RNA酶进行抑制，防止模板RNA被降解[1]。而在PCR扩增时，用一对引物反转录和扩增的特异性并不高，因此大都采用套式PCR，以获得特异性好的PCR产物。这就使得实验成本增高，耗费时间，
In the traditional RT-PCR method, the template RNA is extracted and purified to remove RNase and other impurities, and the possible inhibition of RNase to prevent the template RNA from being degraded [1]. In PCR amplification, using a pair of primers reverse transcription and amplification of specificity is not high, so most of the use of nested PCR to obtain specific good PCR products. This makes the experimental costs increase, time-consuming,