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温室内测试及筛选对银合欢木虱强致病性虫生真菌,发现白僵菌(Beauveria b-assiana)之致病率达90％以上,而灰色拟青霉菌(paecilomyces javanicus)次之,致病率在52～64％之间,白僵菌与灰色拟青霉菌之孢子发芽最适温度分别为24～28℃及28～32℃,又灰色拟青霉菌之孢子于pH2.98～6.1间,发芽均十分良好,但白僵菌则仅于pH5.7～6.1间孢子发芽较好。最适产孢量与培养基质之探讨,结果发现白僵菌以添加蛋白胨或异蛋白胨产孢量可达2.4×10~9孢子/克为最好,灰色拟青霉菌添加几丁质孢子量高达9.8×10~9孢子/克,若仅
In greenhouse, the pathogenicity of Beauveria b-assiana was over 90%, and that of Paecilomyces javanicus was the second. The prevalence of spore germination of Beauveria bassiana and Paecilomyces griseus were 24 ~ 28 ℃ and 28 ~ 32 ℃ respectively, and the spores of Paecilomyces griseus were between pH 2.9 ~ 6.1 , Germination are very good, but Beauveria bassiana only spores germination is better at pH5.7 ~ 6.1. The optimum spore yield and culture medium were studied. The results showed that the best sporulation rate of Beauveria bassiana was 2.4 × 10 ~ 9 spores / g with added peptone or iso-peptone, 9.8 × 10 ~ 9 spores / g, if only