在盆栽条件下,研究了啤酒糟和白酒糟对连作平邑甜茶幼苗生长及其连作土壤中微生物的影响。结果表明,连作土壤添加酒糟可显著提高平邑甜茶幼苗株高、干样质量、鲜样质量等生物量指标。添加酒糟后土壤中细菌、真菌数量与连作土对照相比也有明显提升。通过聚类分析、主成成分分析和多样性分析发现,加入酒糟后土壤中微生物群落结构发生明显变化,且不同酒糟类型和不同酒糟处理方式造成微生物的群落结构不同;由于酒糟自身携带及灭菌酒糟在土壤中滋生的微生物,促使土壤中优势种群的出现,改变了土壤中微生物群落的多样性指数和丰富度指数。对土壤中的层出镰孢菌(Fusarium proliferatum)进行实时荧光定量分析显示,与连作土壤对照中的层出镰孢菌基因拷贝数相比,啤酒糟、白酒糟、灭菌啤酒糟和灭菌白酒糟处理分别降低了39.1%、58.1%、20.9%和39.2%,酒糟灭菌后减弱了对有害菌层出镰孢菌的抑制作用。综上,苹果连作土壤中加入酒糟后,改变了土壤中微生物的群落结构,降低了有害真菌的数量,减轻了苹果连作障碍现象,其中白酒糟效果更好。 Under potted condition, the effects of brewer’s grains and white distiller’s grains on the growth and the microorganisms in the continuous cropping of Malus hupehensis Rehd. The results showed that the addition of distiller ’s grains in continuous cropping soil could significantly improve biomass, such as plant height, dry sample quality and fresh sample quality. The amount of bacteria and fungi in the soil after adding the stillage was also significantly improved compared with that of the control. Through cluster analysis, principal component analysis and diversity analysis, it was found that the microbial community structure changed significantly after the addition of distiller’s grains, and the microbial community structure was different due to different types of distiller’s grains and different ways of stemming treatment. Microorganisms that distiller’s grains breed in the soil promote the emergence of dominant populations of soil and change the diversity index and richness index of soil microbial communities. Real-time fluorescence quantitative analysis of Fusarium proliferatum in soil showed that brewer’s lees, lees distiller’s grains, sterile brewer’s grains and sterilizing Distillers’ grains were reduced by 39.1%, 58.1%, 20.9% and 39.2%, respectively, and the inhibition of distiller’s grains weakened the inhibition of Fusarium spp. In summary, the addition of distiller’s grains to the continuous cropping of apples changed the community structure of microorganisms in the soil, reduced the number of harmful fungi and reduced the cropping obstacle of apple.