制备动物抗血清是免疫病理工作中必不可少的环节。尤其是在目前免疫组织化学技术广泛开展,对抗血清种类的需要不断增加,而国内尚缺乏成套商品供应的情况下,自已动手制备抗血清更显得重要。传统的注射抗原的部位有皮下、皮内、肌肉、腹腔、静脉和局部淋巴结等.这些方法有的操作复杂,有的需要时间太长,有的则不能产生满意的抗体效价。我们根据冯元春的报道并经我们的实验证实,睑结膜注射血清抗原是一种制备抗血清的较好方法。 Preparation of animal antisera is an essential part of immunopathology. Especially in the current immunohistochemistry widely carried out, increasing the need for anti-serum species, but the domestic supply of complete sets of products is still lacking circumstances, self-prepared anti-serum more important. Traditional injection of antigens are subcutaneous, intradermal, intramuscular, intraperitoneal, intravenous and local lymph nodes, etc. Some of these methods are complicated to operate and sometimes take too long, while others do not produce satisfactory antibody titer. According to Feng Yuanchun’s report and confirmed by our experiments, the conjunctival injection of serum antigens is a better way to prepare antisera.