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  5. Cloning,expression and purification of the ligand-binding region of human IL-6R in E.coli and its pr

Cloning,expression and purification of the ligand-binding region of human IL-6R in E.coli and its pr

来源 :中国科学：化学英文版 | 被引量 : 0次 | 上传用户：fly8468
【摘 要】
：
The ligand-binding region of human IL-6R is taken as the target gene fragment to be clonedand expressed.With pET-3b as expressing vector,two recombinants pET-6R
【作 者】
：
段聚宝 王嘉玺 韩家淮 彭善云 邹民吉 苗继红 赵春文 马贤 
【机 构】
：
InstituteofBasicMedicalSciences,TheScrippsResearchInstitute
【出 处】
：
中国科学：化学英文版
【发表日期】
：
1995年11期
【关键词】
：
HUMAN IL-6 receptor CLONING expression structure function E.COLI human IL-6 rece 
【基金项目】
：
the National Natural Scienoe Foundation of China
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The ligand-binding region of human IL-6R is taken as the target gene fragment to be clonedand expressed.With pET-3b as expressing vector,two recombinants pET-6R(B)and pET-6R(B)4 have beenconstructed encoding the ligand-binding region(28 kD)of hIL-6R and i
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