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[目的]分析1998—2007年厦门地区从水和水产品中分离的220株O1群霍乱弧菌非产毒株的型别分布。[方法]220株菌株的基因组DNA经NotⅠ酶切、脉冲场电泳后,利用Bionumerics软件分析电泳图谱。[结果]以90.0%为标准,220株菌株可分成50种不同的脉冲场电泳技术(PFGE)型,相同来源的同一批菌株属于相同PFGE型别;不同来源或不同年份的菌株的PFGE型存在差异,但部分菌株属于相同PFGE型。[结论]水和水产品污染的霍乱弧菌O1群非产毒株趋异性高,牛蛙养殖池与周围环境存在交叉污染;脉冲场电泳技术有助于对霍乱的日常监测。
[Objective] To analyze the type distribution of 220 strains of Vibrio cholerae O1 non-producing strains isolated from water and aquatic products in Xiamen from 1998 to 2007. [Method] The genomic DNA of 220 strains was digested by NotⅠ and then subjected to pulsed field electrophoresis. The electrophoresis pattern was analyzed by Bionumerics software. [Result] With 90.0% as the standard, 220 strains could be divided into 50 different types of PFGE and the same strains from the same source belonged to the same PFGE type. PFGE types existed in different sources or years However, some strains belong to the same PFGE type. [Conclusion] The non-producing strains of Vibrio cholerae O1 group polluted by water and aquatic products have high divergence and cross contamination with the surrounding environment. Pulsed-field electrophoresis is helpful for routine monitoring of cholera.