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本文旨在研究脑组织中氨基酸含量与新生大鼠缺氧缺血性脑病(HIE)发病机制的关系及脑活素的治疗机制,在建立新生大鼠HIE模型的基础上,采用氨基酸自动分析仪检测103例大鼠左侧顶叶脑组织氨基酸含量,并研究脑组织病理改变,同时通过侧脑室及肌肉两种途径给予脑活素(2.5mL·kg~(-1))治疗,每日一次,观察72h。结果显①HIE组脑组织兴奋性氨基酸(EAA)(谷氨酸、天门冬氨酸)含量明显增高,P<0.01,以缺氧缺血后48h EAA含量最高;②脑活素治疗组在侧脑室及肌肉注射脑活素治疗48h时,EAA含量均明显低于同期各HIE对照组,差异显著,P<0.01。与正常对照组比较,治疗后24h及48h存在差异(P<0.01),治疗72h后差异无显著意义(P>0.01)。③侧脑室及肌肉注射脑活素后24h,两组脑组织中EAA含量差异无显著意义(P>0.05);48h后,两组出现差异,P<0.05。结论①脑组织兴奋性氨基酸含量异常升高与缺氧缺血性脑病的发病机制有一定关系。②脑活素治疗HIE的机制可能与其显著降低脑组织中EAA含量有关。③侧脑室注射脑活素和肌肉注射脑活素的治疗效果存在一定的差异。
The purpose of this paper is to study the relationship between the amino acid content in brain tissue and the pathogenesis of neonatal hypoxic-ischemic encephalopathy (HIE) and the therapeutic mechanism of cerebrolysin. On the basis of establishing the neonatal rat model of HIE, an automatic amino acid analyzer The contents of amino acids in the left parietal lobe of 103 rats were detected and the histopathological changes of the brain were observed. Cerebrolysin (2.5 mL · kg -1) was administrated intracerebroventricularly and intramuscularly once daily , Observe 72h. The results showed that the content of EAA (glutamic acid, aspartic acid) in brain tissue of HIE group was significantly increased (P <0.01), and the content of EAA was the highest 48 h after hypoxia-ischemia; (2) And intramuscular injection of cerebrolysin 48h, EAA levels were significantly lower than the same period HIE control group, significant difference, P <0.01. Compared with the normal control group, there was a significant difference at 24h and 48h after treatment (P <0.01). There was no significant difference after 72h treatment (P> 0.01). (3) 24h after intracerebroventricular and intramuscular injection of cerebrolysin, there was no significant difference in EAA content between the two groups (P> 0.05). After 48h, there was a significant difference between the two groups (P <0.05). Conclusions ① The abnormal increase of excitatory amino acid in brain tissue is related to the pathogenesis of hypoxic-ischemic encephalopathy. ② The mechanism of cerebrolysin treatment of HIE may be related to its significant reduction in EAA content in brain tissue. ③ lateral ventricular injection of cerebrolysin and intramuscular injection of cerebrolysin treatment there are some differences.