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目的探讨骨髓基质细胞(bone marrow stromal cells,BMSCs)移植治疗对缺氧缺血性大鼠学习记忆能力的影响及相关机制。方法取SD大鼠BMSCs进行培养,BrdU标记,经侧脑室植入7日龄缺氧缺血性大鼠模型中,8周后进行Morris水迷宫实验,水迷宫实验后取大鼠脑组织进行固定、脱水、包埋、切片;免疫组化行海马区神经元特异烯醇化酶标记及BrdU检测。结果 Morris水迷宫实验显示模型组大鼠T1和T2分别较假手术组大鼠缩短(P<0.05);细胞组大鼠T1和T2分别较模型组大鼠延长(P<0.05)。NSE免疫组化显示模型组大鼠海马阳性细胞积分光密度值(integral optical density,IOD)较假手术组IOD值减小(P<0.05),细胞组IOD值较模型组明显增加(P<0.05),细胞组BrdU免疫组化结果未见阳性细胞。结论 BMSCs经侧脑室植入可改善大鼠的学习记忆能力;其机制可能为注入侧脑室的BMSCs及相关物质促进自体神经元的再生。
Objective To investigate the effects of bone marrow stromal cells (BMSCs) transplantation on learning and memory in hypoxic-ischemic rats and its related mechanisms. Methods BMSCs of SD rats were cultured and labeled with BrdU. Morphologically, the rats were subjected to Morris water maze test after 8 weeks and then subjected to water maze test. , Dehydrated, embedded, sliced; immunohistochemistry of hippocampus neuron-specific enolase markers and BrdU detection. Results Morris water maze test showed that T1 and T2 in model group were shorter than those in sham operation group respectively (P <0.05); T1 and T2 in cell group were longer than model group (P <0.05). NSE immunohistochemistry showed that the integral optical density (IOD) of hippocampal positive cells in model group was lower than that in sham operation group (P <0.05), and the IOD value in cell group was significantly higher than that in model group (P <0.05) ), BrdU immunohistochemistry showed no positive cells in the cell group. Conclusion BMSCs implanted into the lateral ventricle can improve learning and memory in rats. The mechanism may be that BMSCs injected into the lateral ventricle and related substances promote the regeneration of autonomic neurons.