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对牛蒡苷元 (ACT)的钙拮抗作用进行探讨 ,为确认 ACT是牛蒡子解表功能的有效成分提供实验依据。描记给 ACT前后 ,各标本对 KCl或 Ca Cl2 诱发收缩的量效曲线 ,按 Scott法计算 PD’2 ;测定给 ACT后 ,标本对乙酰胆硷 (Ach)诱发的两相收缩的抑制百分率。 ACT对离体大鼠气管、结肠、肺动脉、胸主动脉平滑肌由 KCl引起的收缩产生非竞争性拮抗作用 ,其 PD’2 分别为 4.0 1,5 .11,5 .98,6 .0 5 ;ACT和维拉帕米 (Verapamil)相似 ,对离体豚鼠气管平滑肌由 Ca Cl2 引起的收缩产生非竞争性拮抗作用 ,其 PD’2 分别为 4.0 4,5 .6 2 ;对 Ach诱发的两相收缩只明显抑制第一时相收缩 ,抑制率分别为 6 6 .14% ,81.42 % .ACT对平滑肌的松弛作用可能是由于阻滞电压依赖性钙通道和内钙释放所致 ;ACT是牛蒡子解表功能的有效成分。
Calcium antagonism of Astragaloside (ACT) was explored to provide experimental basis for confirming that ACT is an effective component of Arctium lappa. Trace the dose-response curves for the induced contractions of KCl or CaCl2 before and after ACT, calculate PD’2 by Scott’s method, and determine the percentage of inhibition of acetylcholinesterone (Ach)-induced biphasic contractions after giving ACT. ACT exerted non-competitive antagonism on the contraction of the trachea, colon, pulmonary artery, and thoracic aorta smooth muscle induced by KCl in rats, and the PD’2 was 4.01, 5.11.1, 5.98, and 6.05, respectively. Similar to Verapamil, ACT produced a non-competitive antagonistic effect on the CaCl2-induced contraction of the tracheal smooth muscle in isolated guinea pigs, with a PD’2 of 4.04 to 5.62, respectively; and two phases evoked by Ach. The contraction only significantly inhibited the first phase contraction, and the inhibitory rates were 66.14% and 81.42%, respectively. The relaxation effect of .ACT on smooth muscle might be due to the blockage of voltage-dependent calcium channels and release of intracellular calcium; ACT was calf The active ingredients of the solution table function.