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本试验选用巴西固氮甘蔗品种B 1和B 8为供试材料,在温室条件下桶栽砂培甘蔗施以含不同钼水平的营养液(0m g/L、0.01m g/L、0.02m g/L、0.04m g/L),在不同时期测定甘蔗叶片中硝酸还原酶、G S酶活性及硝态氮、氨态氮含量。试验结果表明,在大棚含氮条件下,三个钼处理都提高了两个甘蔗品种的硝酸还原酶活性而降低了硝态氮含量。0.01m g/L钼处理在拔节期后提高了B 1叶片的G S酶活性,而在幼苗期提高了B 8叶片的G S酶活性,降低了氨态氮含量,促进了氮代谢。在大棚无氮条件下,三个钼处理在拔节期后都提高了两个甘蔗品种的NR酶活性,降低硝态氮含量;提高了B 1的G S酶活性,降低氨态氮含量;在分蘖期也提高了B 8叶片的G S酶活性而降低了其氨态氮含量。因而在大棚里,无论是含氮或无氮条件下,在不同时期不同钼处理对甘蔗氮代谢和氨同化过程的作用也不同,但是相对以较低水平钼处理较能提高两个甘蔗品种的硝酸还原酶活性而降低硝态氮含量;提高了B 1与B 8的G S酶活性,降低氨态氮含量,促进了甘蔗的氮代谢和氨同化。
In this experiment, Brazilian nitrogen-fixing sugar cane varieties B 1 and B 8 were selected as the tested materials. Under the greenhouse conditions, barley-cultivated sugarcane plants were treated with different levels of molybdenum (0 mg / L, 0.01 mg / L, 0.02 mg / L , 0.04mg / L). The contents of nitrate reductase, GSase, nitrate nitrogen and ammonia nitrogen in sugarcane leaves were determined at different stages. The results showed that the three molybdenum treatments increased the nitrate reductase activity of two sugarcane varieties and decreased the content of nitrate nitrogen in the greenhouse under the condition of nitrogen. 0.01 mol / L molybdenum treatment increased G 1 enzyme activity of B 1 leaves at jointing stage, but increased G 2 enzyme activity of B 8 leaves at seedling stage, decreased ammonia nitrogen content and promoted nitrogen metabolism. Under the condition of no nitrogen in the greenhouse, the three molybdenum treatment increased the NR activity of two sugarcane varieties after jointing, decreased the content of nitrate nitrogen, increased the activity of GS of B 1 and decreased the content of ammonia nitrogen, The stage also increased the GSase activity of B 8 leaves and decreased its ammonia nitrogen content. Therefore, in the greenhouse, the effect of different treatments of molybdenum on nitrogen metabolism and ammonia assimilation of sugar cane in different periods was different under either nitrogen or nitrogen-free conditions, but relatively lower levels of molybdenum treatment could improve the sugar content of two sugarcane varieties Nitrate reductase activity and decreased the content of nitrate nitrogen. The activities of GSase of B 1 and B 8 were increased, the ammonia nitrogen content was decreased, and the nitrogen metabolism and ammonia assimilation of sugarcane were promoted.