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目的：探讨胰岛素作用下，牙周膜（ＰＤＬ）细胞碱性磷酸酶（ＡＬＰ）活性和总蛋白含量的变化。方法：细胞生物学法、酶动力学法和考马斯亮蓝法。结果：胰岛素浓度超过１０－４Ｕ／ｍｌ时，明显增加ＰＤＬ细胞的ＡＬＰ活性和总蛋白含量（Ｐ＜０．０１）。其中，在１０－４～１０－１Ｕ／ｍｌ之间，效应随浓度增加而增加，超过１０－１Ｕ／ｍｌ效应趋于稳定，此时效应最大，ＡＬＰ活性和总蛋白含量分别比对照组增加１．４倍和１．２倍。结论：胰岛素明显促进ＰＤＬ细胞分化及蛋白合成功能。胰岛素作为骨代谢的重要调节因子，可能对牙周组织的再生修复有调节作用。
Objective: To investigate the changes of alkaline phosphatase (ALP) activity and total protein in periodontal ligament (PDL) cells under the action of insulin. Methods: Cell biology, enzyme kinetics and Coomassie brilliant blue method. Results: When insulin concentration exceeded 10-4U / ml, the ALP activity and total protein content of PDL cells were significantly increased (P <0.01). Among 10-4 U / ml, the effect increased with the increase of concentration, while the effect of more than 10-1 U / ml tended to be stable, the effect was maximal, ALP activity and total protein content increased by 1 .4 times and 1.2 times. Conclusion: Insulin can significantly promote PDL cell differentiation and protein synthesis. Insulin as an important regulator of bone metabolism may have regulative effects on the regeneration of periodontal tissue.