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目的:利用体外细胞试验评价羟基喜树碱纳米晶体hydroxycamptothecin nanocrystals,HCPT-NCs)抗肿瘤作用及其机制。方法:以羟基喜树碱注射液(hydroxycamptothecin injection,HCPT-I)对肝癌SMMC-7721细胞的干预作用作为对照,应用MTT法检测羟基喜树碱纳米晶体对肝癌细胞SMMC-7721细胞的增殖抑制作用,流式细胞术分析细胞凋亡率,RT-PCR方法检测survivin基因表达的变化。结果:与对照制剂HCPT-I相比,HCPT-NCs对SMMC-7721肿瘤细胞生长抑制和杀伤作用,促凋亡能力和对survivin基因靶向作用,有显著性差异(p 0.05)。结论:HCPT-NCs对SMMC-772细胞的生长有较明显的抑制作用,促进SMMC-7721细胞凋亡可能和下调survivin的表达有关,和HCPT-I相比作用明显。
OBJECTIVE: To evaluate the anti-tumor effect and mechanism of hydroxycamptothecin nanocrystals (HCPT-NCs) in vitro by cell assay. Methods: The effect of HCPT-I on the hepatocarcinoma SMMC-7721 cells was compared. MTT assay was used to detect the inhibitory effect of hydroxycamptothecin nanocrystals on the proliferation of SMMC-7721 cells , The apoptosis rate was analyzed by flow cytometry, and the change of survivin gene expression was detected by RT-PCR. Results: Compared with HCPT-I, the HCPT-NCs had significant difference (P <0.05) on the growth inhibition and killing effect, apoptosis-promoting ability and survivin gene targeting of SMMC-7721 tumor cells. Conclusion: HCPT-NCs can significantly inhibit the growth of SMMC-772 cells and promote the apoptosis of SMMC-7721 cells, which may be related to the down-regulation of survivin expression.