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目的:观察不同情况下结核性胸腔积液中3种致炎细胞因子IL-12、IL-18、TNF-α和2种抑制性细胞因子TGF-β1、IL-10的变化。方法:采用ABC-ELISA及双抗体夹心ELISA进行检测,并进行相关性分析。结果:除IL-18外,IL-12、TNF-α、IL-10胸水中含量都远远超过血清中的含量,TGF-β1血清中含量远远超过胸水中含量(P<0.05或P<0.01)。结论:IL-18可能用作加强疫苗保护性的一种辅助成分;证明细胞因子IL-12、TNF-α、TGF-β1、IL-10与结核病免疫发病密切相关。
Objective: To observe the changes of three inflammatory cytokines IL-12, IL-18, TNF-α and two inhibitory cytokines TGF-β1 and IL-10 in tuberculous pleural effusion under different conditions. Methods: ABC-ELISA and double antibody sandwich ELISA were used for detection and correlation analysis. Results: The levels of IL-12, TNF-α and IL-10 in pleural effusion far exceeded those in serum except IL-18. The content of TGF-β1 in serum significantly exceeded that in pleural effusion (P <0.05 or P < 0.01). Conclusion: IL-18 may be used as an adjunct to enhance vaccine protection. It is demonstrated that the cytokines IL-12, TNF-α, TGF-β1 and IL-10 are closely related to the pathogenesis of tuberculosis.