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目的:观察四逆汤饼灸治疗兔膝关节退变模型治疗前后血清IL-1、IL-6和TNF-α及软骨形态的变化,探讨四逆汤饼灸治疗膝骨性关节炎的作用机理。方法:将36只雄性新西兰大耳兔随机分为四逆汤饼灸组、安慰剂饼灸组和模型组,每组12只,采用右后肢伸直位固定法建立兔膝关节退变模型,运用双抗体夹心酶联免疫吸附法(ELISA)检测造模前、治疗前和治疗后四逆汤饼灸组、安慰剂饼灸组和模型组兔血清中IL-1、IL-6和TNF-α的水平,参考彭太平法观察膝关节软骨形态学变化。结果:治疗前各组较模型兔血清中IL-1、IL-6和TNF-α的浓度均显著升高(P<0.05);治疗2周后,大体观察模型组膝关节软骨变薄,出现明显的关节软骨损伤,四逆汤饼灸组膝关节软骨结明显改善(P<0.05);较模型组,四逆汤饼灸组、安慰剂饼灸组血清IL-1、IL-6和TNF-α水平明显降低(P<0.05);较安慰剂饼灸组,四逆汤饼灸组血清IL-1、IL-6和TNF-α水平降低,差异有统计学意义(P<0.05)。结论:四逆汤饼灸能修复KOA的软骨损伤,其机制可能与降低IL-1、IL-6和TNF-α水平有关。
Objective: To observe the changes of serum IL-1, IL-6, TNF-α and cartilage morphology before and after treatment of Sini decoction moxibustion in treating rabbit knee joint degeneration model and to explore the mechanism of action of Sini decoction moxibustion on knee osteoarthritis . Methods: Thirty-six male New Zealand big-eared rabbits were randomly divided into Sini decoction moxibustion group, placebo moxibustion group and model group, with 12 rats in each group. Rabbit knee joint degeneration model was established by extension of right hind limbs. The levels of IL-1, IL-6 and TNF-α in sera of Sini Decoction moxibustion group, placebo moxibustion group and model group before and after treatment were measured by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) α level, with reference to Peng Taiping method observation of knee joint cartilage morphological changes. Results: Before treatment, the concentrations of IL-1, IL-6 and TNF-α in the serum of the model rabbits in each group were significantly increased (P <0.05). After 2 weeks of treatment, the articular cartilage of the model group became thinner and appeared Compared with model group, Sini Decoction moxibustion group and placebo group moxibustion group, serum IL-1, IL-6 and TNF (P <0.05). The levels of IL-1, IL-6 and TNF-α in serum in placebo moxibustion group and Sini Decoction moxibustion group were significantly lower than those in placebo group (P <0.05). Conclusion: Sini Decoction moxibustion can repair KOA cartilage injury, the mechanism may be related to the reduction of IL-1, IL-6 and TNF-α levels.