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实验观察了兔角膜碱烧伤后角膜超氧化物歧化酶和电子自旋共振波谱的变化。角膜碱烧伤模型为中央区角膜,直径9毫米,2当量浓度的氢氧化钠,烧伤60秒钟。实验结果表明,烧伤后第3、7和15天,角膜超氧化物歧化酶活性较正常角膜显著下降,P<0.01。电子自旋共振波谱发现,烧伤后的角膜组织中有过氧化自由基存在(g值为2.0106±0.0027)。实验结果提示:超氧自由基及其代谢产物可能是造成碱烧伤后组织病理损伤的因素之一。
The changes of corneal superoxide dismutase and electron spin resonance spectroscopy were observed after corneal alkali burn in rabbits. Corneal alkali burn model for the central cornea, 9 mm in diameter, 2 equivalents of sodium hydroxide, burn for 60 seconds. The experimental results showed that corneal superoxide dismutase activity decreased significantly on the 3rd, 7th and 15th day after burn compared with that of the normal cornea (P <0.01). Electron spin resonance spectroscopy found that there was peroxide radical in corneal tissue after burns (g value is 2.0106 ± 0.0027). The experimental results suggest that superoxide radical and its metabolites may be one of the factors that cause histopathological damage after alkali burn.