目的　从基因水平了解 6例Castleman’s病 (CD)病理细胞组成特点。方法　肿物切除以后 ,苏木精 伊红染色作常规组织病理检查以明确肿物的组织学类型 ;应用免疫组化法研究细胞表型 ,明确肿物的细胞组成 ;根据细胞组成 ,应用RT PCR和克隆测序方法了解肿物主要细胞克隆组成。结果　 6例患者皆为限局性透明血管型CD ;肿瘤组织滤泡样结构中主要为B淋巴细胞 ,滤泡间隙为T淋巴细胞。RT PCR扩增产物经聚丙烯酰胺凝胶电泳后得到单一条带 ,RT PCR产物克隆测序得到12 8bp、12 2bp两种碱基长度的克隆 ,相同长度序列间具有高度一致性 ,克隆内分别存在 12个和 7个碱基的差异。结论　 6例患者肿瘤组织中含有单 /寡克隆瘤性B细胞 ,这些B细胞起源于生发中心细胞。 Objective To understand the histopathological features of 6 Castleman’s disease (CD) genes at the gene level. Methods After resection of the tumor, hematoxylin and eosin staining was used for routine histopathological examination to clarify the histological type of the tumor. Immunohistochemistry was used to study the phenotype of the tumor and the cell composition of the tumor was determined. RT PCR And clone sequencing methods to understand the main cell clones of the tumor. Results The 6 patients were all limited by the transparent vascular CD; tumor tissue follicular structure is mainly B lymphocytes, follicular gap for T lymphocytes. A single band was obtained after polyacrylamide gel electrophoresis (RT-PCR). The PCR products were cloned and sequenced to obtain clones of 12 8 bp and 12 2 bp in length. The results showed that there was a high degree of homology between the two sequences with the same length, Differences between 12 and 7 bases. CONCLUSION: Six patients had tumor cells containing oligoclonal clonogenic B cells. These B cells originated from germinal center cells.