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目的:探讨白细胞介素17(IL-17)与慢性乙型肝炎病毒(HBV)相关性肝病特别是乙肝肝纤维化发生的相关性。方法:酶联免疫吸附试验(ELISA)及逆转录-多聚酶链反应(RT-PCR)法分别测定36例慢乙肝、42例肝硬化(Child-pughA、B、C分别为15、12、15例)、34例原发性肝癌、30例慢性重型乙型肝炎及20例健康对照者血清中的IL-17和外周血单个核细胞(PBMC)中IL-17mRNA水平及肝硬化Child-pugh分级患者血清中的IL-17和肝纤维化指标IV型胶原(C-IV),层黏连蛋白(LN),透明质酸(HA)的浓度。结果:与正常对照组相比,血清IL-17和PBMC中IL-17mRNA水平在4组病人中表达升高(P<0.01);与其他各组相比,IL-17及mRNA水平在肝硬化中升高尤其明显(P<0.01)。肝硬化患者血清IL-17及肝纤维化指标水平在Child-pughC组高于Child-pughA和Child-pughB组,Child-pughB组高于Child-pughA组(P<0.01)。IL-17水平与Child-pugh分级评分及C-IV,LN,HA浓度均呈正相关(r=0.582、0.568、0.682、0.764,P<0.01)。结论:IL-17在慢性HBV相关性肝病尤其是乙肝肝硬化中表达明显升高,IL-17可能与慢性HBV相关性肝病尤其是乙肝肝纤维化的发生有明显的相关性。
Objective: To investigate the correlation between interleukin-17 (IL-17) and chronic hepatitis B virus (HBV) -related liver diseases, especially hepatitis B liver fibrosis. Methods: 36 cases of chronic hepatitis B and 42 cases of liver cirrhosis (Child-pughA, B, C, 15, 12, 15 cases respectively) were determined by ELISA and RT- ), 34 cases of primary liver cancer, 30 cases of chronic severe hepatitis B and 20 cases of healthy controls IL-17 and peripheral blood mononuclear cells (PBMC) IL-17mRNA levels and cirrhosis Child-pugh grading patients Serum levels of IL-17 and indicators of hepatic fibrosis type IV collagen (C-IV), laminin (LN), and hyaluronic acid (HA) Results: Compared with the normal control group, the levels of IL-17 mRNA in serum IL-17 and PBMC increased in 4 groups (P <0.01). Compared with other groups, the levels of IL-17 and mRNA in cirrhosis Especially in the middle (P <0.01). The levels of serum IL-17 and liver fibrosis in patients with cirrhosis were higher in Child-pughC group than those in Child-pughA and Child-pughB groups, but higher in Child-pughB group than in Child-pughA group (P <0.01). IL-17 levels were positively correlated with Child-pugh score and C-IV, LN and HA concentrations (r = 0.582,0.568,0.682,0.764, P <0.01). Conclusion: The expression of IL-17 in chronic HBV-related liver disease, especially hepatitis B cirrhosis, is significantly increased. IL-17 may be significantly correlated with chronic HBV-related liver disease, especially hepatitis B liver fibrosis.