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[目的]探讨双环铂对人卵巢癌细胞A2780的凋亡诱导作用并初步探索其凋亡机制。[方法]MTT法观察双环铂对A2780细胞增殖的抑制作用,荧光显微镜观察细胞形态学变化,多参数流式细胞术及TUNEL法检测细胞凋亡率,并测定caspase-3的活性变化及cas-pase抑制剂对细胞存活率的影响。[结果]双环铂可抑制A2780细胞的增殖,IC50332.9±26.3μmol/L。双环铂剂量为1×IC50、2×IC50、4×IC50时,诱导A2780细胞凋亡率分别为18.5%±5.4%、39.7%±6.0%和63.7%±3.4%,呈明显的剂量依赖性。经双环铂作用后漂浮细胞呈现典型凋亡细胞形态学改变。caspase-3活性随着细胞凋亡率增加而增加,泛caspase抑制剂z-VAD-fmk部分抑制细胞死亡。[结论]双环铂可体外诱导A2780细胞凋亡,其凋亡途径存在caspase依赖性及可能存在非caspase依赖性途径。
[Objective] To investigate the apoptosis-inducing effect of Bcl-2 on human ovarian cancer cell line A2780 and to explore its apoptosis mechanism. [Methods] The inhibitory effect of Bcl-2 on the proliferation of A2780 cells was observed by MTT assay. The morphological changes of cells were observed by fluorescence microscopy. The apoptosis rate was detected by multi-parameter flow cytometry and TUNEL assay. The caspase-3 activity and cas- Effect of pase inhibitor on cell viability. [Results] Bcl-2 inhibited the proliferation of A2780 cells with IC50332.9 ± 26.3μmol / L. The apoptotic rate of A2780 cells induced by Bcl-2 was 18.5% ± 5.4%, 39.7% ± 6.0% and 63.7% ± 3.4%, respectively, in a dose-dependent manner with 1 × IC50,2 × IC50 and 4 × IC50. Floated cells showed morphological changes of typical apoptotic cells. The activity of caspase-3 increased with the increase of apoptosis rate, while z-VAD-fmk, a pan caspase inhibitor, partially inhibited cell death. [Conclusion] Bcl-2 can induce the apoptosis of A2780 cells in vitro, and the apoptotic pathway is caspase-dependent and may have a non-caspase-dependent pathway.