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目的:分析载脂蛋白B(apolipoprotein B,apo B)基因C7673T多态性与长沙地区汉族人群脑出血的关系及其对血脂水平的影响。方法:采用聚合酶链式反应-限制性片段长度多态性分析法(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)和DNA直接测序法检测130例脑出血患者和100例正常对照者的apoB基因C7673T多态;并用氧化酶法测定其血清甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白(high density lipoprotein-cholesterol,HDL)、低密度脂蛋白(low density lipoprotein-cholesterol,LDL)水平;酶联免疫吸附法测定脂蛋白(a)[lipoprotein(a),LP(a)]浓度;免疫比浊法测定apoB100及apoAI浓度。结果:(1)脑出血组和对照组apoB基因C7673T多态T等位基因频率分别为0.108和0.040,脑出血患者组apoB基因C7673T多态T等位基因频率显著高于对照组(P<0.01)。(2)脑出血组apoB基因C7673T多态T/C基因型较C/C基因型的TC和LDL-C显著增高,而HDL-C显著降低(P<0.05),其它指标差异无统计学意义(P>0.05)。结论:apoB基因C7673T多态可能与长沙地区汉族人群脑出血有关,且可能通过改变血脂水平影响脑出血的发生发展。
Objective: To analyze the relationship between C7673T polymorphism of apolipoprotein B (apo B) gene and cerebral hemorrhage in Han nationality in Changsha area and its effect on serum lipids. Methods: 130 cases of cerebral hemorrhage and 100 cases of normal control were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA direct sequencing The apoB gene C7673T polymorphism was detected by enzyme linked immunosorbent assay (ELISA). The levels of triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL), low density lipoprotein low density lipoprotein-cholesterol and low-density lipoprotein-cholesterol (LDL) were detected by enzyme linked immunosorbent assay (ELISA). The concentrations of lipoprotein (a), LP Results: (1) The allele frequencies of C7673T polymorphism of apoB gene in cerebral hemorrhage group and control group were 0.108 and 0.040, respectively. The frequency of C allele C7673T polymorphism in cerebral hemorrhage patients was significantly higher than that in control group (P <0.01) ). (2) The C7673T polymorphism T / C genotype of apoB gene was significantly higher than that of C / C genotype in cerebral hemorrhage group, while HDL-C was significantly lower (P <0.05), but there was no significant difference in other indexes (P> 0.05). Conclusion: ApoB gene C7673T polymorphism may be related to cerebral hemorrhage in Han population in Changsha area, and it may affect the occurrence and development of cerebral hemorrhage by changing blood lipid level.