目的为研究低氧诱导因子1(HIF1)在抗氧化剂加强低氧细胞毒性的过程中所起的作用。方法低氧敏感的Hep3B细胞以低氧模拟物氯化钴(0·1mmol/L)预处理2h,换新鲜培养液,分成2组,分别以氧化剂(H2O2与GSSG)和抗氧化剂(H2O2酶与GSH)处理24h,收获细胞后提取总蛋白,采用Western Blot分析H Objective To investigate the role of hypoxia inducible factor 1 (HIF1) in the process of antioxidants to enhance hypoxic cytotoxicity. Methods Hypoxia-sensitive Hep3B cells were pretreated with 0.5% cobalt chloride (0.1 mmol / L) for 2 h and replaced with fresh medium. The cells were divided into two groups. The oxidative agents (H2O2 and GSSG) and antioxidants GSH) for 24 h, and the total protein was extracted after the cells were harvested. H was analyzed by Western Blot