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为探讨一氧化氮 (NO)和一氧化氮合酶 (NOS)在粉尘致病中的作用 ,利用改良Myvirk法收集兔肺泡巨噬细胞 (AM)进行体外培养 ,采用硝酸还原酶法测定二氧化钛、石英尘和新康温石棉处理AM后培养上清液中亚硝酸根 /硝酸根 (NO-2 /NO-3 )的含量 (NO的静态氧化形式 ) ,同时利用酶 底物反应形成有色物质测定AM的细胞裂解液中NOS的酶活性。结果显示 ,随粉尘浓度的增加 :(1)温石棉组和石英组的AM死亡率增高 ,且温石棉组 >石英组 >二氧化钛组 ;(2 )温石棉组和石英组NO-2 /NO-3 的含量增加 ,呈剂量反应关系 (P <0 0 5 ) ,在高剂量组 ,两组NO均高于二氧化钛组 ,温石棉组和石英组之间无显著性差别 (P >0 0 5 ) ;(3)石英和温石棉均使NOS活性随剂量增加而升高 ,但高剂量石棉组的NOS活性降低 ,这可能是AM死亡率增高所致 ;(4 )石英和温石棉介导的NO和NOS活性变化具有一致性。提示NO和NOS在石英和石棉相关疾病中具有潜在的作用
To investigate the role of nitric oxide (NO) and nitric oxide synthase (NOS) in pathogenesis of dust, rabbit alveolar macrophages (AM) were collected by modified Myvirk method and cultured in vitro. Nitric acid reductase The contents of nitrite / nitrate (NO-2 / NO-3) in the culture supernatant (static oxidized form of NO) after quartz dust and fresh Kangweng asbestos treatment were measured, and the reaction of enzyme substrate to form the colored substance Enzyme activity of NOS in cell lysates of AM. The results showed that with the increase of dust concentration, (1) the mortality rate of AM was higher in chrysotile and quartz groups than in quartz and chrysotile groups; (2) (P <0 05). In the high dose group, NO of both groups was higher than that of titanium dioxide group. There was no significant difference between chrysotile and quartz group (P> 0.05) ; (3) Both quartz and chrysotile have NOS activity increased with the increase of dose, but the NOS activity of high dose asbestos group decreased, which may be caused by the increase of AM mortality; (4) And NOS activity changes consistent. Suggesting that NO and NOS have a potential role in quartz and asbestos-related diseases