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目的 :研究PCR SSCP技术检测原发性胃腺癌石蜡标本中p53第 7外显子基因突变 ,探讨其与肿瘤发展的关系。方法 :应用PCR SSCP(聚合酶链反应 单链构象多态性 )技术检测 37例原发性胃腺癌标本中p53第 7外显子的基因突变 ,同时应用免疫组织化学技术检测p53蛋白的表达。探讨其与肿瘤发展的关系。结果 :37例原发性胃腺癌标本中 ,p53第 7外显子基因突变率为 19% (7/ 37) ,7例发生突变的标本 ,其p53蛋白表达亦呈阳性 ,低分化腺癌突变率高于高、中分化腺癌 (P =0 0 0 0 6 ) ,发生淋巴结转移组突变率高于未发生淋巴结转移组 (P =0 0 0 0 3)。结论 :临床检测胃腺癌原发灶中是否存在p53第 7外显子基因突变 ,有助于识别高度恶性的胃腺癌。
Objective: To study the mutation of p53 exon 7 gene in paraffin embedded specimens of primary gastric adenocarcinoma by PCR SSCP and to explore its relationship with tumor development. Methods: The gene mutation of exon 7 of p53 in 37 cases of primary gastric adenocarcinoma was detected by PCR SSCP, and the expression of p53 protein was detected by immunohistochemistry. Explore its relationship with tumor development. Results: In 37 specimens of primary gastric adenocarcinoma, the mutation rate of p53 exon 7 gene was 19% (7/37). The specimens of 7 patients with mutation of p53 gene were also positive for p53 protein. The mutation of poorly differentiated adenocarcinoma The rate of mutation in lymph node metastasis group was higher than that in non-lymph node metastasis group (P = 0.00006). Conclusion: It is helpful to detect the mutation of p53 exon 7 in the primary tumor of gastric adenocarcinoma, which is helpful to identify the highly malignant gastric adenocarcinoma.