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目的:探讨热放疗对舌鳞癌Tca8113细胞和耐药的Tca8113/CBDEA细胞耐药性的影响。方法:实时定量逆转录PCR(RT-PCR)检测热放疗对Tca8113细胞和耐药的Tca8113/CBDEA细胞多药耐药基因1(MDR1)、多药耐药相关蛋白1基因(MRP1)和谷胱甘肽硫-转移酶π基因(GST-π)表达的影响和检测热放疗对细胞内阿霉素浓度的影响。结果:Tca8113/CBDEA和Tca8113细胞热放疗后耐药基因MDR1的表达在4h和24h无明显改变(P>0.05)。MRP1基因的表达在4h和24h组均有明显下降(P<0.05),GST-π基因表达在4h组Tca8113和Tca8113/CBDEA的表达无明显改变(P>0.05),在24h组有明显下降(P<0.05).热放疗以后肿瘤细胞内阿霉素(ADM)浓度有明显上升。结论:热放疗联合应用抑制了放疗造成的耐药基因表达上升,增加了细胞内的药物浓度,热放疗联用不会促使肿瘤细胞产生MDR。
Objective: To investigate the effects of thermal radiotherapy on the drug resistance of Tca8113 cells and drug-resistant Tca8113 / CBDEA cells. Methods: RT-PCR was used to detect the expression of MDR1, MRP1, Effects of heat-treated radiotherapy on the intracellular adriamycin concentration and the effect of GST-π expression. Results: The expression of multidrug resistance gene MDR1 in Tca8113 / CBDEA and Tca8113 cells after heat-radiotherapy did not change significantly at 4h and 24h (P> 0.05). The expression of MRP1 gene in both 4h and 24h groups was significantly decreased (P <0.05). The expression of GST-π gene was not significantly changed in 4h group (P> 0.05), but significantly decreased in 24h group P <0.05) .After radiotherapy, the concentration of doxorubicin (ADM) in tumor cells increased significantly. Conclusion: The combination of thermal radiotherapy and radiotherapy can inhibit the increase of drug resistance gene induced by radiotherapy and increase the concentration of intracellular drug. The combination of radiotherapy and radiotherapy does not promote the production of MDR in tumor cells.