论文部分内容阅读
本研究以不同高度的慈竹笋和当年生慈竹为材料,采用酶联免疫法和转录组测序技术,对慈竹笋生长过程中内源激素含量动态积累和脱落酸(ABA)、赤霉素(GA)与生长素(IAA)KEGG代谢途径相关基因差异表达进行分析。结果表明,慈竹在竹笋整个生长过程中,ABA、GA和IAA总含量随着株高的增高呈现不同的变化趋势,GA总含量变化呈“S”型曲线,IAA总含量变化呈双峰曲线,而ABA含量变化不明显;KEGG代谢途径中相关基因存在差异表达,其中ABA代谢途径中PSY和BCH在笋50 cm时呈现上调表达,ZE在150 cm时呈现下调表达。GA代谢途径中KAO与CPS在早期呈现上调表达,后呈现下调表达。IAA代谢途径中CATB在笋生长到50 cm呈现上调表达,而后呈现下调表达的趋势,而ALDH和OGDC随着笋的伸长呈现表达上调。
In this study, the bamboo shoots with different heights and the current year-born bamboo were used as materials. The dynamic changes of endogenous hormones, abscisic acid (ABA), gibberellin GA) and auxin (IAA) KEGG metabolic pathway related genes were analyzed. The results showed that the total content of ABA, GA and IAA showed different trend with the increase of plant height during the whole growth period of Phyllostachys praecox, the content of total GA changed “S” curve, and the content of IAA changed in double Peak curve, while ABA content did not change significantly. The related genes in KEGG metabolic pathway were differentially expressed. Among them, PSY and BCH in ABA metabolic pathway were up-regulated at 50 cm shoots and ZE at 150 cm down-regulated. In the GA metabolic pathway, KAO and CPS were up-regulated in the early stage and down-regulated afterwards. IAA metabolic pathway of CATB up to 50 cm in the shoots showed up-regulated expression, and then showed a downward trend in expression, and ALDH and OGDC showed up-regulation of shoot elongation.