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目的 研究中子不同剂量照射人鼻咽癌（ＣＮＥ２） 细胞凋亡发生特点及与Ｘ射线所致凋亡的差异。探讨凋亡在中子治疗肿瘤中的作用及临床意义。方法 采用琼脂糖凝胶电泳及ＤＮＡ特异性荧光染色方法（Ｈｏｅｃｈｓｔ３３３４２） 检测照射后不同时间人鼻咽癌（ＣＮＥ２）细胞。结果 发现中子可诱导人鼻咽癌细胞发生凋亡，这种凋亡发生存在着一定的时间剂量相关性。在相同剂量照射下，同一时间点上，中子照射所致的凋亡反应强于Ｘ 射线所致的凋亡。结论 快中子照射离体细胞可引起较强的凋亡反应。中子杀伤肿瘤的机理可能也是主要通过凋亡途径来实现的。
Objective To study the characteristics of apoptosis of CNE2 irradiated by different doses of neutron and its difference with apoptosis induced by Xray. To explore the role of apoptosis in neutron therapy of tumor and its clinical significance. Methods Human nasopharyngeal carcinoma (CNE-2) cells were detected by agarose gel electrophoresis and DNA-specific fluorescent staining (Hoechst33342). The results showed that neutrons can induce apoptosis in human nasopharyngeal carcinoma cells, and there is a certain time-dose-dependent apoptosis. Under the same dose of irradiation, the apoptosis induced by neutron irradiation was stronger than that caused by X-ray at the same time point. Conclusion Fast neutron irradiation in vitro cells can cause strong apoptotic response. The mechanism by which neutrons kill tumors may also be mainly through the apoptotic pathway.