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目的观察重组人白介素 11(recombinanthumaninterleukin 11,rhIL 11)对亚致死剂量γ射线照射所致急性放射病猕猴血细胞减少的治疗作用。方法 19只正常成年猕猴用60 Coγ射线源一次全身照射 3 0Gy,分照射对照、rhIL 1130 ,6 0和 12 0 μg·kg-1·d-14组。照后当天开始每天一次皮下注射给药 ,连续 14d。结果 rhIL 11可明显升高照射猴外周血小板、白细胞数最低值 ,缩短其最低值的持续时间 ,加速恢复。药后 1周内 ,各给药组外周血红细胞数较照射对照组均有不同程度的降低 ,从药后第 19天开始则明显高于照射对照组。照射后 30和 45d骨髓细胞培养结果表明 ,rhIL 11可明显刺激照射猴骨髓有核细胞体外形成CFU MK、CFU Mix、CFU E和BFU E的能力。病理组织学观察结果同样表明 ,rhIL 11治疗组骨髓粒细胞和巨核细胞明显增加。结论 rhIL 11可明显促进急性放射病猴骨髓造血功能的恢复 ,在本实验 3个剂量组中 ,6 0 μg组的效果较 30和 12 0 μg组好。我们认为 ,rhIL 11在急性放射病和肿瘤患者大剂量放、化疗后造血功能的重建中具有良好的应用前景。
Objective To observe the therapeutic effect of recombinant human interleukin 11 (rhIL 11) on blood cell cytopenia induced by sub-lethal radiation dose of γ-ray in rhesus monkeys with acute radiation sickness. METHODS: Nineteen normal adult macaques were irradiated with 60 Co γ-rays once in a total dose of 30 Gy, divided into control, rhIL 1130, 60 and 120 μg·kg-1·d-14 groups. On the day after the start of treatment, subcutaneous injections were administered once a day for 14 days. Results rhIL 11 significantly increased the peripheral blood platelet and leukocyte counts of monkeys, shortened the duration of the lowest value, and accelerated recovery. Within one week after drug administration, the number of red blood cells in the peripheral blood of the drug-administered groups decreased compared with the control group, and it was significantly higher than the irradiated control group from the 19th day after the drug administration. At 30 and 45 days after irradiation, the results of myeloid cell culture indicated that rhIL 11 could significantly stimulate the ability of irradiated bone marrow nucleated cells to form CFU MK, CFU Mix, CFU E and BFU E in vitro. Histopathological observations also showed that rhIL 11 treatment significantly increased bone marrow granulocytes and megakaryocytes. Conclusion rhIL 11 can significantly promote the recovery of bone marrow hematopoietic function in monkeys with acute radiation sickness. In the three dose groups of the experiment, the effect of the 60 μg group was better than that of the 30 and 120 μg groups. We believe that rhIL 11 has a good application prospect in the reconstruction of hematopoietic function after radiation and chemotherapy in patients with acute radiation sickness and cancer.