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目的检测儿童癫痫患者外周T淋巴细胞活化状态以及细胞因子水平变化。方法选取20名儿童癫痫患者和19名健康对照儿童,采集外周血,分离外周血单个核细胞(PBMC),用细胞膜表面标记抗体流式细胞术检测T淋巴细胞表面共刺激分子CD69、CD25和细胞毒性T淋巴细胞相关蛋白4(CTLA4)的表达,用细胞内因子染色结合流式细胞术检测T细胞细胞因子γ干扰素(IFN-γ)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)和IL-17A的表达,利用细胞内因子染色结合流式细胞术检测调节性T淋巴细胞(Treg)表达IL-10的情况。结果与对照组相比,儿童癫痫患者外周血中T淋巴细胞表面高表达共刺激分子CD69、CD25和CTLA4,活化的CD4+T细胞高表达IFN-γ、TNF-α、IL-6和IL-17 A。在儿童癫痫患者高表达IL-10的Treg数目增加。结论儿童癫痫患者外周T淋巴细胞活化并产生细胞因子。
Objective To detect peripheral T lymphocyte activation and cytokine levels in children with epilepsy. Methods Twenty children with epilepsy and 19 healthy control children were enrolled in this study. Peripheral blood was collected and peripheral blood mononuclear cells (PBMCs) were isolated. The expression of costimulatory molecules CD69, CD25 and cell surface on T lymphocytes were detected by flow cytometry T lymphocyte associated protein 4 (CTLA4) was detected by flow cytometry. T cell cytokines IFN-γ, IL-6 and TNF-α were detected by intracellular factor staining and flow cytometry. α (TNF-α) and IL-17A, and the expression of IL-10 in regulatory T lymphocytes (Treg) was detected by intracellular factor staining combined with flow cytometry. Results Compared with the control group, the expression of costimulatory molecules CD69, CD25 and CTLA4 on the surface of T lymphocytes in peripheral blood of children with epilepsy were higher than those in the control group. The expressions of IFN-γ, TNF-α, IL-6 and IL- 17 A. The number of Tregs that are highly expressed in children with epilepsy is increased. Conclusion Peripheral T lymphocytes are activated and cytokines are produced in children with epilepsy.