全血和血浆是最常用于诊断疾病的标本。但由于在血液标本中存在着抑制PCR的因子,因此,在进行PCR之前,一般都要仔细纯化血液标本中的核酸。经典的从临床血液标本中纯化核酸的方法所涉及的一些试剂和步骤,如有机溶剂、变性剂、去污剂以及浓缩、沉淀,也对随后的PCR分析产生影响。因此,建立一种简便易行的血液标本的制备方法,是很有意义的。 Whole blood and plasma are the most commonly used specimens for the diagnosis of disease. However, since there are factors that inhibit PCR in blood samples, nucleic acids in blood samples should generally be carefully purified prior to PCR. Some of the reagents and procedures involved in classical methods of purifying nucleic acids from clinical blood samples, such as organic solvents, denaturants, detergents, and concentrates, precipitate, also have an impact on subsequent PCR analysis. Therefore, it is significant to establish a simple and convenient method of preparing blood samples.