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目的:探讨新疆维吾尔族妇女子宫颈鳞癌血浆中死亡相关蛋白激酶(DAPK)基因启动子甲基化水平。方法:使用血浆游离DNA提取试剂盒提取血浆DNA,用甲基化修饰试剂盒修饰DNA,使用甲基化特异性PCR法检测血浆中DAPK基因启动子甲基化水平。结果:44例维吾尔族妇女子宫颈鳞癌患者中,有18例DAPK基因启动子甲基化,27例未发现DAPK基因启动子甲基化,甲基化率为40%(18/45);40例健康对照者检测出DAPK基因启动子甲基化阳性2例,DAPK基因启动子甲基化阴性38例,甲基化率为5%(2/40)。结论:血浆中DAPK基因启动子甲基化对诊断维吾尔族妇女子宫颈鳞癌有潜在的价值。
Objective: To investigate the promoter methylation of the death-associated protein kinase (DAPK) gene in the cervical squamous cell carcinoma of Uighur women in Xinjiang. Methods: Plasma DNA was extracted by plasma free DNA extraction kit, DNA was modified by methylation modified kit, and methylation-specific PCR was used to detect the promoter methylation level of DAPK gene in plasma. Results: Among 44 Uighur women with cervical squamous cell carcinoma, 18 cases had DAPK gene promoter methylation, 27 cases did not find DAPK gene promoter methylation, and the methylation rate was 40% (18/45). Two cases of DAPK gene promoter methylation were detected in 40 healthy controls, while 38 cases were negative for DAPK gene promoter methylation. The methylation rate was 5% (2/40). Conclusion: The promoter methylation of DAPK gene in plasma has potential value in the diagnosis of cervical squamous cell carcinoma in Uighur women.