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目的:探讨补阳还五汤对脑缺血再灌注大鼠第10号染色体缺失的磷酸酶和张力蛋白同源物基因(PTEN)mRNA表达与血小板活化的影响。方法:将无特定病原体(SPF)级SD大鼠60只随机分为假手术组、模型组、氢氯吡格雷组、补阳还五汤高、低剂量组,每组12只。氢氯吡格雷组ig给予氯吡格雷6.8 mg·kg-1·d-1,补阳还五汤高、低剂量组分别ig给予补阳还五汤26,6.5 g·kg-1·d-1,其余各组ig给予生理盐水,连续给药14 d后,采用大脑中动脉线栓法复制大鼠急性局灶性脑缺血再灌注模型,并在造模前2 h给予相应ig处理,造模60 min后拔除栓线再灌注12 h后收集相应标本保存,测定各组大鼠血浆P-选择素(CD62P)含量与海马组织PTEN mRNA表达情况。结果:与假手术组比较,模型组神经行为评分显著增加,与模型组比较,各用药组的神经行为评分显著降低;与假手术组比较,模型组血浆中CD62P表达显著升高(P<0.05);与模型组比较,补阳还五汤组与氢氯吡格雷组均能显著抑制血浆中CD62P表达(P<0.05),但补阳还五汤组与氢氯匹格雷组之间抑制作用无显著性差异;与假手术组比较,模型组PTEN mRNA表达显著增加;与模型组比较,各用药组的PTEN mRNA表达显著降低(P<0.05),以补阳还五汤高剂量组尤为明显。结论:补阳还五汤对急性脑缺血再灌注损伤模型大鼠的保护机制可能与下调PTEN基因过表达及抑制CD62P表达有关。
Objective: To investigate the effect of Buyang Huanwu decoction on the expression of phosphatase and tensin homolog (PTEN) mRNA on platelet 10 and platelet activation in cerebral ischemia-reperfusion rats. Methods: Sixty SD rats without specific pathogen (SPF) were randomly divided into sham operation group, model group, clopidogrel group and high and low doses of Buyang Huanwu decoction group, 12 rats in each group. Hydrochloride-clopidogrel group given clopidogrel 6.8 mg · kg-1 · d-1, Buyang Huanwu Decoction high and low dose group were given ig Buyanghuanwu 26,6.5 g · kg-1 · d- 1, the other groups were given ig saline, continuous administration of 14 d, using the middle cerebral artery occlusion rat focal cerebral ischemia-reperfusion model and given 2 h before modeling the corresponding ig treatment, Sixty minutes after model establishment, the plugs were removed and re-injected for 12 hours. The specimens were collected and the levels of plasma P-selectin (CD62P) and PTEN mRNA in hippocampus were determined. Results: Compared with the sham operation group, the neurobehavioral score of the model group increased significantly. Compared with the model group, the neurobehavioral score of each drug group decreased significantly. Compared with the sham operation group, the expression of CD62P in the model group was significantly increased (P <0.05 ). Compared with the model group, the expression of CD62P in plasma was significantly inhibited by Buyang Huanwu Decoction group and hydrochloropirimine group (P <0.05), but there was no difference between Buyang Huanwu Decoction group and hydrochloropigment group (P <0.05). Compared with the sham operation group, the expression of PTEN mRNA in the model group was significantly increased. Compared with the model group, the expression of PTEN mRNA in each group was significantly decreased (P <0.05), especially in the high-dose BYHWD group . Conclusion: The mechanism of Buyang Huanwu Decoction on the protection of acute cerebral ischemia-reperfusion injury in rats may be related to the down-regulation of PTEN gene expression and the inhibition of CD62P expression.