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以灭活溶藻弧菌（０．０５％甲醛，３０℃，８ｈ）制成菌苗，注射免疫实验兔获得抗血清。该 抗血清经吸附后与副溶血弧菌、河流弧菌等１０株对照菌株无交叉反应。用该抗血清进行溶藻 弧菌ＥＬＩＳＡ检测，其最低检测极限为９７０００ｃｆｕ／ｍｌ。将该方法应用于养殖现场，检测结果为：海 水中溶藻弧菌浓度均低于最低检测限、外观健康大黄鱼肝脏中溶藻弧菌的阳性检出率为 ２０％、患病大黄鱼的阳性检出率为８０％，表明ＥＬＩＳＡ法不仅可以用于诊断大黄鱼的溶藻弧菌 病，也可以检测无病症带菌大黄鱼。
To inactivate Vibrio alginolyticus (0.05% formaldehyde, 30 ℃, 8h) made of vaccine, immune experimental rabbits were injected antiserum. The antiserum did not cross-react with 10 control strains such as Vibrio parahaemolyticus and Vibrio cholerae after adsorption. Vibrio alginolyticus ELISA test using this antiserum, the minimum detection limit of 97000cfu / ml. The method was applied to the breeding site, the test results were as follows: the concentrations of V. alginolyticus in sea water were below the minimum detection limit, the positive detection rate of V. alginolyticus in the liver of healthy large yellow croaker was 20% Positive detection rate was 80%, indicating that ELISA method can be used not only for the diagnosis of large albacterium alginolyticus disease, can also be detected without disease-carrying large yellow croaker.